Chemometec NC-100 Manuel d'utilisateur

NucleoCounter® User's Guide P/N 991-0009 Revision 1.2 Technology that counts

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Introduction and intended use x Introduction and intended use The NucleoCounter® is a novel instrument designed specially to count mammalian cells

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Warnings and precautions xii Warnings and precautions Wherever the symbol appears on the NucleoCounter instrument, it indicates that the manual m

Warnings and precautions xiii Table Table Table Table 2222 Description of the NucleoCounter Printer Output connector Pin noPin noPin noPin no

Warnings and precautions xiv The user shall be made aware of that, if the NucleoCounter and the external power supply is used in a manner not spec

Warnings and precautions xv Never pipette by mouth. Avoid cross contamination of the samples when preparing of the samples. This can compromise the qu

1 Inspection and Unpacking of Equipment 1 Table of contents DECLARATION OF CONFORMITY ...

1 Inspection and Unpacking of Equipment 2 5.4.3 Setting Date (F200) and Time (F201) ... 18 5.4.4

1 Inspection and Unpacking of Equipment 3 1 Inspection and Unpacking of Equipment Upon receiving the order from ChemoMetec A/S, the box or boxes sho

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2 Introducing the NucleoCounter 5 2 Introducing the NucleoCounter The NucleoCounter method provides a fast alternative to the manual techniques, yiel

2 Introducing the NucleoCounter 6 2.1.1 Fluorescence microscope The compact fluorescence microscope integrated in the NucleoCounter comprises LED´s (l

2 Introducing the NucleoCounter 7 A fluorescent image of the content of the measurement chamber is thus achieved. Example of such an image is shown in

2 Introducing the NucleoCounter 8 Figure Figure Figure Figure 5555 The NucleoCassette. The fluorescent dye, propidium iodide, is immobilized in the f

2 Introducing the NucleoCounter 9 by multiplying the imaged area with the depth of the cassette. The imaged area is only dependent on the optics of th

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3 Control Buttons, Keypad and Display 11 3 Control Buttons, Keypad and Display 3.1 Interactive Controls 3.1.1 Control Buttons There are four Control

3 Control Buttons, Keypad and Display 12 3.2 Interface Display 3.2.1 Status indicator A small Status Indicator light above the “On/Off” button (see F

4 Installation and Start-up 13 4 Installation and Start-up 4.1 Power on, power off The NucleoCounter is powered by a 12VDC external power supply. To

ii NucleoCounterMammalian Cell CounterP / N 9 9 1 - 0 0 0 9 ( E n g l i s h )R e v i s i o n 1 . 2N o v e m b e r , 2 0 1 3

4 Installation and Start-up 14 After a successful start-up the display changes (Figure 9) and the status indicator on the keyboard panel turns green.

5 Operation of the NucleoCounter 15 5 Operation of the NucleoCounter 5.1 Ready mode After the NucleoCounter has started successfully the display sho

5 Operation of the NucleoCounter 16 After analysis the lid is lifted and the cassette is removed from the insertion slit and disposed of according to

5 Operation of the NucleoCounter 17 5.3.3 External computer If an external computer with the NucleoView software installed on it is connected, the res

5 Operation of the NucleoCounter 18 • Press <↵>. 5.4.2 Saving parameters The representation of the results is changed with effect from the next

5 Operation of the NucleoCounter 19 5.4.5 LCD contrast (F220) When typing “F + 2 + 2 + 0 + F + 2 + 2 + 0 + F + 2 + 2 + 0 + F + 2 + 2 + 0 + ↵↵↵↵” it i

5 Operation of the NucleoCounter 20 in line 3. The serial number of the instrument is shown in line 4 of the display. The display text is almost ident

5 Operation of the NucleoCounter 21 Press “↵↵↵↵” to continue. Then a “Wait” message appears in the display: wait...wait...wait...wait...

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6 Total and viability count 23 6 Total and viability count This chapter describes the basic procedures for the determination of the total concentrati

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6 Total and viability count 24 available to load the NucleoCassette. It is important that the sample must be representative of the cell suspension. Th

6 Total and viability count 25 After approximately 30 seconds the NucleoCounter shows the result in the display and on the computer if one is connecte

6 Total and viability count 26 6.3 Calculate viability When both the concentration of non-viable cells and the total concentration of cells are known

6 Total and viability count 27 buffer added to the samples are altered. Note that it is important to add equal volumes of Lysis buffer and Stabilizing

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7 Maintenance of NucleoCounter 29 7 Maintenance of NucleoCounter Cleaning on a regular basis of the NucleoCounter is recommended in order to protect

7 Maintenance of NucleoCounter 30 Figure Figure Figure Figure 25252525... An image showing a contamination of the optical system, visible as a

7 Maintenance of NucleoCounter 31 7.1.4 Spill of liquid If liquid has been spilled on the instrument it can contaminate elements of the optical system

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8 Troubleshooting - Error messages 33 8 Troubleshooting - Error messages Under certain conditions the NucleoCounter will display error messages durin

ivCaution!Caution!Caution!Caution! This equipment must be operated as described in this User’ s Guide. Please read the entire guide before attemp

8 Troubleshooting - Error messages 34 8.3 Actuator error messages The actuator moves the piston down into the barrel of the cassette. Two different a

8 Troubleshooting - Error messages 35 Sensor errorSensor errorSensor errorSensor error Refer to manualRefer to manualRefer to manualRefer to m

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9 Technical specifications 37 9 Technical specifications 9.1 The NucleoCounter Specificity The NucleoCounter counts mammalian cell nuclei stained w

9 Technical specifications 38 INSTALLATION CATEGORY II (Refer to UL 61010A-1) OVERVOLTAGE CATEGORY II (Refer to UL 61010A-1) POLLUTION DEGREE II

10 Equipment and Accessories 39 10 Equipment and Accessories 10.1 Equipment and Accessories List The equipment and accessories for the NucleoCounter

10 Equipment and Accessories 40 Item Part no at ChemoMetec A/S Description current. Mains Power cord See section below. A cord for mains supply of

10 Equipment and Accessories 41 10.3 Mains Power cord Detachable power supply cord set for the Class I specified power supplies: For US 125 Vac UL li

10 Equipment and Accessories 42 The item can be obtained from Kenro Ltd, Greenbridge Road, Swindon SN3 3LH, UK. Tel: +44 (0) 1793 615836, E-mail: sale

10 Equipment and Accessories 43 Table Table Table Table 4444 Description of the NucleoCounter Printer Output connector Pin noPin noPin noPin no

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10 Equipment and Accessories 44 package insert of the NucleoCounter test kit for operation and information on how to apply the kit in a test of a Nucl

11 Appendix 1: Result mode 4 45 11 Appendix 1: Result mode 4 The table below provides an overview of the results displayed when mode 4 (statistical ta

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12 Appendix 2: Extended NucleoCounter® NC-100TM Settings 47 12 Appendix 2: Extended NucleoCounter® NC-100TM Settings Enabling of extended instrument

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49 13 Appendix 3: WEEE directive information in more EU languages United Kingdom Correct Disposal of This Product (Waste Electrical & Electroni

50 Norway Korrekt avhending av dette produkt (Avfall elektrisk og elektronisk utstyr) - Kun Europa Denne merkingen som vises på produktet eller de

51 Finland Tämän tuotteen turvallinen hävittäminen (elektroniikka ja sähkölaitteet) - Vain Eurooppa Oheinen merkintä tuotteessa tai tuotteen oheis

52 The information contained herein is to the best of our knowledge accurate and complete. However cell species and c

vi Declaration of Conformity Name of product: NucleoCounter Type: Mammalian Cells Other identifying data: Part no. 900-0004 The pr

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viii WEEE directive information – Europe only Correct Disposal of This Product (Waste Electrical & Electronic Equipment) - Europe only This mar